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Literature summary for 2.7.7.71 extracted from
- General assay for enzymes in the heptose biosynthesis pathways using electrospray ionization mass spectrometry (2017), Appl. Microbiol. Biotechnol., 101, 4521-4532 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene hddC or wcbM, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Burkholderia pseudomallei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (-)-nyasol | - |
Burkholderia pseudomallei | |
| additional information | inhibitor screening | Burkholderia pseudomallei |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state kinetics | Burkholderia pseudomallei | |
| 0.16 | - |
GTP | recombinant enzyme, pH 7.5, 37°C | Burkholderia pseudomallei |  |
| 0.33 | - |
D-glucose 1-phosphate | recombinant enzyme, pH 7.5, 37°C | Burkholderia pseudomallei | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Burkholderia pseudomallei | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glycero-alpha-D-manno-heptose 1-phosphate + GTP | Burkholderia pseudomallei | - |
GDP-D-glycero-alpha-D-manno-heptose + diphosphate | - |
? | |
| D-glycero-alpha-D-manno-heptose 1-phosphate + GTP | Burkholderia pseudomallei K96243 | - |
GDP-D-glycero-alpha-D-manno-heptose + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia pseudomallei | Q63R79 | - |
- |
| Burkholderia pseudomallei K96243 | Q63R79 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography | Burkholderia pseudomallei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 1-phosphate + GTP | - |
Burkholderia pseudomallei | GDP-D-glucose + diphosphate | - |
? | |
| D-glucose 1-phosphate + GTP | - |
Burkholderia pseudomallei K96243 | GDP-D-glucose + diphosphate | - |
? | |
| D-glycero-alpha-D-manno-heptose 1-phosphate + GTP | - |
Burkholderia pseudomallei | GDP-D-glycero-alpha-D-manno-heptose + diphosphate | - |
? | |
| D-glycero-alpha-D-manno-heptose 1-phosphate + GTP | - |
Burkholderia pseudomallei K96243 | GDP-D-glycero-alpha-D-manno-heptose + diphosphate | - |
? | |
| additional information | the assay is performed with glucose 1-phosphate and GTP | Burkholderia pseudomallei | ? | - |
- |
|
| additional information | the assay is performed with glucose 1-phosphate and GTP | Burkholderia pseudomallei K96243 | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-glycero-alpha-D-manno-heptose-1-phosphate guanylyltransferase | - |
Burkholderia pseudomallei |
| HddC | - |
Burkholderia pseudomallei |
| WcbM | - |
Burkholderia pseudomallei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Burkholderia pseudomallei |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0107 | - |
GTP | recombinant enzyme, pH 7.5, 37°C | Burkholderia pseudomallei | |
| 0.0117 | - |
D-glucose 1-phosphate | recombinant enzyme, pH 7.5, 37°C | Burkholderia pseudomallei | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Burkholderia pseudomallei |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0176 | - |
recombinant enzyme, pH 7.5, 37°C, versus 1-phosphate and GTP | Burkholderia pseudomallei | (-)-nyasol |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the GDP-D-alpha-D-heptose biosynthesis pathway (GDP-6dD-heptose pathway) includes D-glycero-alpha-D-manno-heptose-7-phosphate kinase (HddA or WcbL) and D-glycero-alpha-D-manno-heptose-1-phosphate guanylyltransferase (HddC or WcbM) instead of D-glycero-beta-D-manno-heptose-7-phosphate kinase (HldA) and D-glycero-beta-D-manno-heptose-1-phosphate adenylyltransferase (HldC), respectively. Identification of the activity of each enzyme in the GDP-6dD-heptose biosynthesis pathway by MS analysis, overview | Burkholderia pseudomallei |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.036 | - |
D-glucose 1-phosphate | recombinant enzyme, pH 7.5, 37°C | Burkholderia pseudomallei | |
| 0.067 | - |
GTP | recombinant enzyme, pH 7.5, 37°C | Burkholderia pseudomallei | |
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